I signed up for HuggingFace and successfully created my token, I attempted to fetch the SOD1 sequence (ID: P00441) from UniProt and manually introduced the A4V mutation as required. Initially I struggled due to repeated errors. but I kept on trying and was able to run all the peptides.

My Token value: **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

Mutated sequence of SOD1**

MATKVVCVLKGDGPVQGIINFEEQKESNGPVKVWGSIKGLTEGLHGFHVHEFGDNTAGCTSAGPHFNPLSRKHGGPKDEERHVGD
LGNVTAKDGVADVSIEDSVISLSGDHCIIGRTLVVHEKADDLGKGGNEESTKTGNAGSRLACGVIGIAQ

4 peptides of 12 AA size generated

Four peptides generated were

WHSYATALEWKK WLSGPVGLAWWE WRYYVTALEHWX WRYGAVAAELWX

One additional peptide was provided in the homework as well from https://genesdev.cshlp.org/content/22/11/1451

 FLYRWLPSRRGG 

Generating peptide-sequence multimers on Alphafold2

Here is the Alphafold analysis for all peptide fragments:

Peptide 1

WHSYATALEWKK 

Peptide 2