Part 1: Benchling & In-silico Gel Art

See the Gel Art: Restriction Digests and Gel Electrophoresis protocol for details. Overview:

• [x] Make a free account at benchling.com

• [x] Import the Lambda DNA.

• [x] Simulate Restriction Enzyme Digestion with the following Enzymes: EcoRI HindIII BamHI KpnI EcoRV SacI SaclI

  

• [x] Create a pattern/image in the style of Paul Vanouse’s Latent Figure Protocol artworks.

I was trying to attempt the Japanese waving cat, but could not get her paw right. maybe with some combinations forming thicker bands may help.

Part 2: Gel Art - Restriction Digests and Gel Electrophoresis

Perform the lab experiment you designed in Part 1 and outlined in the Gel Art: Restriction Digests and Gel Electrophoresis protocol.

Could not perform due to lack of lab access

Part 3: DNA Design Challenge

3.1.     Choose your protein.

In recitation, we discussed that you will pick a protein for your homework that you find interesting. Which protein have you chosen and why? Using one of the tools described in recitation (NCBI, UniProt, google), obtain the protein sequence for the protein you chose.

sp|P02144|MYG_HUMAN Myoglobin OS=Homo sapiens OX=9606 GN=MB PE=1 SV=2 GLSDGEWQQVLNVWGKVEADIPGHGQEVLIRLFKGHPETLEKFDKFKHLKSEDEMKASEDLKKHG TVVLTALGAILKKKGHHEAEIKPLAQSHATKHKIPVKYLEFISEAIIHVLHSKHPGDFGADAQG AMTKALELFRNDMAAKYKELGYQG

3.2.     Reverse Translate: Protein (amino acid) sequence to DNA (nucleotide) sequence.